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Regulatory
Part:BBa_K3380103:Design
Designed by: Alexandru Popov Group: iGEM20_Edinburgh (2020-10-10)
T7 strong promoter with ArsR binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25
Design Notes
Usually the ArsR binding site is preceding the promoter sequence, however we placed it after the promoter to block the T7 RNA polymerase transcribing. The promoter and the ArsR binding site had 2 overlapping bp which were fused together.
Source
Originally the ArsR binding site was isolated from the genome of Escherichia coli str. K-12 substrain MG1655. The construct was "de novo" synthesized and purchased.
References
C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.