Regulatory

Part:BBa_K3380103:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-10)


T7 strong promoter with ArsR binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

Usually the ArsR binding site is preceding the promoter sequence, however we placed it after the promoter to block the T7 RNA polymerase transcribing. The promoter and the ArsR binding site had 2 overlapping bp which were fused together.


Source

Originally the ArsR binding site was isolated from the genome of Escherichia coli str. K-12 substrain MG1655. The construct was "de novo" synthesized and purchased.

References

C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.